Application of Scanning Electron Microscopy to Study Morphological and Anatomical Features of Sea Buckthorn Leaves

Introduction. Scanning electron microscopy is a modern method that allows us to study not only the morphological features of objects, but also conduct micro-X-ray structural analysis. Currently, being actively introduced into biological objects (including plant ones). Sea buckthorn ( Hippophaë rhamnoides L.) leaves are non-pharmacopoeial type medicinal raw materials. Further morphology, anatomy and phytochemical composition can contribute production new, including combined, medicines, which will require development pharmacopoeia article for this material. Aim. The aim was anatomical sea by scanning microscopy. Materials methods. object dried whole L.), collected in Voronezh region 2021 during period mass fruit maturity. To carry out microscopy, pieces were previously sprayed with gold on an automatic spraying unit Q150R ES (Quorum Technologies Ltd., United Kingdom) increase conductivity. Micrographs obtained using microscope JSM-6510LV (JEOL Japan). Results their discussion. morphology some structure studied main microdiagnostic signs (surface character, types trichomes, presence stomata) clarified. content elements (silicon, potassium, aluminum, carbon calcium) determined microrentgenostructural pollen grains obtained, element iron them established. Conclusion. For first time, used leaves. diagnostic location have been It has established predominates material elements, calcium accumulates. accumulation silicon potassium numerous hairs densely covering upper, especially lower, surfaces leaf blade assumed. surface buckthorn, having spherical shape spiny shell, Pollen grains, addition carbon, characterized silicon.